Title
InP Nanowire Biosensor with Tailored Biofunctionalization: Ultrasensitive and Highly Selective Disease Biomarker Detection
Author
Janissen, R. (TU Delft BN/Nynke Dekker Lab; University of Campinas; Kavli institute of nanoscience Delft) 
Sahoo, Prasana K. (University of Campinas)
Santos, Clelton A. (University of Campinas)
Da Silva, Aldeliane M. (University of Campinas)
Von Zuben, Antonio A.G. (University of Campinas)
Souto, Denio E.P. (University of Campinas)
Costa, Alexandre D.T. (Carlos Chagas Institute)
Celedon, Paola (Molecular Biology Institute of Paraná)
Zanchin, Nilson I.T. (Carlos Chagas Institute)
Almeida, Diogo B. (University of Campinas)
Oliveira, Douglas S. (University of Campinas)
Date
2017
Abstract
Electrically active field-effect transistors (FET) based biosensors are of paramount importance in life science applications, as they offer direct, fast, and highly sensitive label-free detection capabilities of several biomolecules of specific interest. In this work, we report a detailed investigation on surface functionalization and covalent immobilization of biomarkers using biocompatible ethanolamine and poly(ethylene glycol) derivate coatings, as compared to the conventional approaches using silica monoliths, in order to substantially increase both the sensitivity and molecular selectivity of nanowire-based FET biosensor platforms. Quantitative fluorescence, atomic and Kelvin probe force microscopy allowed detailed investigation of the homogeneity and density of immobilized biomarkers on different biofunctionalized surfaces. Significantly enhanced binding specificity, biomarker density, and target biomolecule capture efficiency were thus achieved for DNA as well as for proteins from pathogens. This optimized functionalization methodology was applied to InP nanowires that due to their low surface recombination rates were used as new active transducers for biosensors. The developed devices provide ultrahigh label-free detection sensitivities ∼1 fM for specific DNA sequences, measured via the net change in device electrical resistance. Similar levels of ultrasensitive detection of ∼6 fM were achieved for a Chagas Disease protein marker (IBMP8-1). The developed InP nanowire biosensor provides thus a qualified tool for detection of the chronic infection stage of this disease, leading to improved diagnosis and control of spread. These methodological developments are expected to substantially enhance the chemical robustness, diagnostic reliability, detection sensitivity, and biomarker selectivity for current and future biosensing devices.
Subject
Biosensor
Chagas Disease
field effect transistor
indium phosphide
nanowire
surface chemistry
To reference this document use:
http://resolver.tudelft.nl/uuid:b7eca67f-c3a8-4925-8865-517533485602
DOI
https://doi.org/10.1021/acs.nanolett.7b01803
Embargo date
2018-09-12
ISSN
1530-6984
Source
Nano Letters: a journal dedicated to nanoscience and nanotechnology, 17 (10), 5938-5949
Bibliographical note
Accepted Author Manuscript
Part of collection
Institutional Repository
Document type
journal article
Rights
© 2017 R. Janissen, Prasana K. Sahoo, Clelton A. Santos, Aldeliane M. Da Silva, Antonio A.G. Von Zuben, Denio E.P. Souto, Alexandre D.T. Costa, Paola Celedon, Nilson I.T. Zanchin, Diogo B. Almeida, Douglas S. Oliveira, More Authors