Print Email Facebook Twitter Peroxygenase-Catalysed Sulfoxidations in Non-Aqueous Media Title Peroxygenase-Catalysed Sulfoxidations in Non-Aqueous Media Author Li, Huanhuan (Xi’an Jiaotong University; Chinese Academy of Sciences) Shen, Qianqian (Xi’an Jiaotong University; Chinese Academy of Sciences) Zhou, Xiaoying (Chinese Academy of Sciences) Duan, Peigao (Xi’an Jiaotong University) Hollmann, F. (TU Delft BT/Biocatalysis) Huang, Yawen (Chinese Academy of Sciences) Zhang, Wuyuan (Chinese Academy of Sciences) Date 2023 Abstract Chiral sulfoxides are valuable building blocks in asymmetric synthesis. However, the biocatalytic synthesis of chiral sulfoxides is still challenged by low product titres. Herein, we report the use of peroxygenase as a catalyst for asymmetric sulfoxidation under non-aqueous conditions. Upon covalent immobilisation, the peroxygenase showed stability and activity under neat reaction conditions. A large variety of sulfides was converted into chiral sulfoxides in very high product concentration with moderate to satisfactory optical purity (e. g. 626 mM of (R)-methyl phenyl sulfoxide in approx. 89 % ee in 48 h). Further polishing of the ee value via cascading methionine reductase A (MsrA) gave>99 % ee of the sulfoxide. The robustness of the enzymes and high product titer is superior to the state-of-the-art methodologies. Gram-scale synthesis has been demonstrated. Overall, we demonstrated a practical and facile catalytic method to synthesize chiral sulfoxides. Subject Asymmetric sulfoxidationBiocatalysisCascadeEnzyme ImmobilisationPeroxygenase To reference this document use: http://resolver.tudelft.nl/uuid:cd33c3c4-60d8-4e12-80f1-fbc67e2c26fa DOI https://doi.org/10.1002/cssc.202301321 ISSN 1864-5631 Source ChemSusChem (Print): chemistry & sustainability, energy & materials, 17 (6) Part of collection Institutional Repository Document type journal article Rights © 2023 Huanhuan Li, Qianqian Shen, Xiaoying Zhou, Peigao Duan, F. Hollmann, Yawen Huang, Wuyuan Zhang Files PDF ChemSusChem_2023_Li_Perox ... _Media.pdf 1.67 MB Close viewer /islandora/object/uuid:cd33c3c4-60d8-4e12-80f1-fbc67e2c26fa/datastream/OBJ/view