Print Email Facebook Twitter High frequency switched-mode stimulation can evoke post synaptic responses in cerebellar principal neurons Title High frequency switched-mode stimulation can evoke post synaptic responses in cerebellar principal neurons Author Van Dongen, M.N. Hoebeek, F.E. Koekkoek, S.K.E. De Zeeuw, C.I. Serdijn, W.A. Faculty Electrical Engineering, Mathematics and Computer Science Department Microelectronics Date 2015-03-06 Abstract This paper investigates the efficacy of high frequency switched-mode neural stimulation. Instead of using a constant stimulation amplitude, the stimulus is switched on and off repeatedly with a high frequency (up to 100 kHz) duty cycled signal. By means of tissue modeling that includes the dynamic properties of both the tissue material as well as the axon membrane, it is first shown that switched-mode stimulation depolarizes the cell membrane in a similar way as classical constant amplitude stimulation. These findings are subsequently verified using in vitro experiments in which the response of a Purkinje cell is measured due to a stimulation signal in the molecular layer of the cerebellum of a mouse. For this purpose a stimulator circuit is developed that is able to produce a monophasic high frequency switched-mode stimulation signal. The results confirm the modeling by showing that switched-mode stimulation is able to induce similar responses in the Purkinje cell as classical stimulation using a constant current source. This conclusion opens up possibilities for novel stimulation designs that can improve the performance of the stimulator circuitry. Care has to be taken to avoid losses in the system due to the higher operating frequency. Subject computational neurosciencepulsatile stimulationswitched-mode operationPurkinje cellspatch-clamp recordingsOA-Fund TU Delft To reference this document use: http://resolver.tudelft.nl/uuid:d54909a9-8a96-46d7-b828-1bb40acf33bd DOI https://doi.org/10.3389/fneng.2015.00002 Publisher Frontiers ISSN 1662-6443 Source Frontiers in Neuroengineering, 8, 2015 Part of collection Institutional Repository Document type journal article Rights (c) 2015 The Author(s)This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). Files PDF Serdijn_2015.pdf 1.57 MB Close viewer /islandora/object/uuid:d54909a9-8a96-46d7-b828-1bb40acf33bd/datastream/OBJ/view