Print Email Facebook Twitter E-MALDI Title E-MALDI: Optimized conditions during electrowetting-enhanced drop drying for MALDI-MS Author Kudina, Olena (University of Twente) Eral, H.B. (TU Delft Intensified Reaction and Separation Systems; Universiteit Utrecht) Mugele, Frieder (University of Twente) Date 2017 Abstract We recently showed that electrowetting-enhanced sample preparation for MALDI-MS (eMALDI) can increase the intensity of the MALDI signal by 2-25 times compared to conventional drop drying by concentrating all the dried sample in a single spot rather than leaving behind a heterogeneous coffee stain pattern. Here, we demonstrate that the eMALDI signal enhancement can be further increased to more than 100x by systematically optimizing the electrowetting actuation frequency and amplitude. This enables 30x signal increase for a peptide standard. Simultaneously drop drying times can be reduced approximately five times by increasing the actuation voltage and/or decreasing the initial drop volume. Subject eMALDIelectrowetting-enhanced drop drying‘coffee stain’ effect suppressionMALDIMALDI signal intensity increase To reference this document use: http://resolver.tudelft.nl/uuid:ebb1c4d9-b6a8-4993-bfd9-a18309f33e01 DOI https://doi.org/10.1002/jms.3934 Embargo date 2018-04-18 ISSN 1076-5174 Source Journal of Mass Spectrometry, 52 (6), 405-410 Bibliographical note Accepted Author Manuscript Part of collection Institutional Repository Document type journal article Rights © 2017 Olena Kudina, H.B. Eral, Frieder Mugele Files PDF Kudina_et_al_2017_Journal ... ometry.pdf 1.31 MB Close viewer /islandora/object/uuid:ebb1c4d9-b6a8-4993-bfd9-a18309f33e01/datastream/OBJ/view