Print Email Facebook Twitter Bacillus subtilis lipase a—lipase or esterase? Title Bacillus subtilis lipase a—lipase or esterase? Author Bracco Garcia, M.P. (TU Delft BT/Biocatalysis) van Midden, Nelleke (Student TU Delft) Arango, Epifanía (Student TU Delft) Torrelo, Guzman (Student TU Delft) Ferrario, Valerio (University of Trieste) Gardossi, Lucia (University of Trieste) Hanefeld, U. (TU Delft BT/Biocatalysis) Date 2020 Abstract The question of how to distinguish between lipases and esterases is about as old as the definition of the subclassification is. Many different criteria have been proposed to this end, all indicative but not decisive. Here, the activity of lipases in dry organic solvents as a criterion is probed on a minimal α/β hydrolase fold enzyme, the Bacillus subtilis lipase A (BSLA), and compared to Candida antarctica lipase B (CALB), a proven lipase. Both hydrolases show activity in dry solvents and this proves BSLA to be a lipase. Overall, this demonstrates the value of this additional parameter to distinguish between lipases and esterases. Lipases tend to be active in dry organic solvents, while esterases are not active under these circumstances. Subject Bacillus subtilis lipase AEsteraseHydrolaseLipaseOrganic solventTransesterificationWater activity To reference this document use: http://resolver.tudelft.nl/uuid:ef0e8d78-7532-4775-b27c-1ea5fbd7f1a2 DOI https://doi.org/10.3390/catal10030308 ISSN 2073-4344 Source Catalysts, 10 (3) Part of collection Institutional Repository Document type journal article Rights © 2020 M.P. Bracco Garcia, Nelleke van Midden, Epifanía Arango, Guzman Torrelo, Valerio Ferrario, Lucia Gardossi, U. Hanefeld Files PDF catalysts_10_00308.pdf 1.41 MB Close viewer /islandora/object/uuid:ef0e8d78-7532-4775-b27c-1ea5fbd7f1a2/datastream/OBJ/view