Print Email Facebook Twitter Hybrid adaptive and computational light-sheet fluorescence microscopy Title Hybrid adaptive and computational light-sheet fluorescence microscopy Author Wilding, D. (TU Delft Team Raf Van de Plas) Pozzi, P. (TU Delft Team Raf Van de Plas) Soloviev, O.A. (TU Delft Team Raf Van de Plas; Flexible Optical B.V.) Vdovin, Gleb (TU Delft Team Raf Van de Plas; Flexible Optical B.V.) Fiolka, Reto (University of Texas Southwestern) Verhaegen, M.H.G. (TU Delft Team Raf Van de Plas) Contributor Bifano, T.G. (editor) Kubby, J. (editor) Gigan, S. (editor) Date 2018 Abstract The light-sheet fluorescence microscopy is an excellent tool for the investigation of large three dimensional microscopy samples at the cellular level, however, the ability to resolve features is strongly affected by the presence of scattering and aberrations. These effects are two fold in light-sheet microscopy, as the illumination path providing the optical sectioning and the fluorescence detection path are both affected by the aberrations in different ways. To overcome these difficulties, we have developed hybrid adaptive optical and computational microscopy techniques to remove the effect of the aberrations in both the excitation and the fluorescence paths of these microscopes. Subject Adaptive opticsdeconvolutionlight-sheetmicroscopy To reference this document use: http://resolver.tudelft.nl/uuid:efc9bccd-b867-4f05-9d89-560d06fa298b DOI https://doi.org/10.1117/12.2287661 Publisher SPIE, Bellingham, WA, USA ISBN 9781510614895 Source Proceedings of SPIE: Adaptive Optics and Wavefront Control for Biological Systems IV, 10502 Event SPIE BIOS, 2018-01-27 → 2018-01-29, San Francisco, United States Series Proceedings of SPIE, 10502 Part of collection Institutional Repository Document type conference paper Rights © 2018 D. Wilding, P. Pozzi, O.A. Soloviev, Gleb Vdovin, Reto Fiolka, M.H.G. Verhaegen Files PDF 1050212.pdf 759.94 KB Close viewer /islandora/object/uuid:efc9bccd-b867-4f05-9d89-560d06fa298b/datastream/OBJ/view