Print Email Facebook Twitter Toward complete oral cavity cancer resection using a handheld diffuse reflectance spectroscopy probe Title Toward complete oral cavity cancer resection using a handheld diffuse reflectance spectroscopy probe Author Brouwer de Koning, Susan G. (Antoni van Leeuwenhoek Ziekenhuis) Baltussen, Elisabeth J.M. (Antoni van Leeuwenhoek Ziekenhuis) Karakullukcu, M. Baris (Antoni van Leeuwenhoek Ziekenhuis) Dashtbozorg, Behdad (Netherlands Cancer Institute) Smit, Laura A. (Antoni van Leeuwenhoek Ziekenhuis) Dirven, Richard (Antoni van Leeuwenhoek Ziekenhuis) Hendriks, B.H.W. (TU Delft Medical Instruments & Bio-Inspired Technology; Philips Research) Sterenborg, Henricus J.C.M. (Antoni van Leeuwenhoek Ziekenhuis; Amsterdam UMC) Ruers, Theo J.M. (University of Twente; Antoni van Leeuwenhoek Ziekenhuis) Date 2018 Abstract This ex-vivo study evaluates the feasibility of diffuse reflectance spectroscopy (DRS) for discriminating tumor from healthy tissue, with the aim to develop a technology that can assess resection margins for the presence of tumor cells during oral cavity cancer surgery. Diffuse reflectance spectra were acquired on fresh surgical specimens from 28 patients with oral cavity squamous cell carcinoma. The spectra (400 to 1600 nm) were detected after illuminating tissue with a source fiber at 0.3-, 0.7-, 1.0-, and 2.0-mm distances from a detection fiber, obtaining spectral information from different sampling depths. The spectra were correlated with histopathology. A total of 76 spectra were obtained from tumor tissue and 110 spectra from healthy muscle tissue. The first- and second-order derivatives of the spectra were calculated and a classification algorithm was developed using fivefold cross validation with a linear support vector machine. The best results were obtained by the reflectance measured with a 1-mm source-detector distance (sensitivity, specificity, and accuracy are 89%, 82%, and 86%, respectively). DRS can accurately discriminate tumor from healthy tissue in an ex-vivo setting using a 1-mm source-detector distance. Accurate validation methods are warranted for larger sampling depths to allow for guidance during oral cavity cancer excision. Subject diffuse reflectance spectroscopylinear support vector machinemachine learningoral cavity cancerresection margin assessmenttissue recognition To reference this document use: http://resolver.tudelft.nl/uuid:f9467d4d-6f95-47df-baba-7443cf4c237a DOI https://doi.org/10.1117/1.JBO.23.12.121611 ISSN 1083-3668 Source Journal of Biomedical Optics, 23 (12) Part of collection Institutional Repository Document type journal article Rights © 2018 Susan G. Brouwer de Koning, Elisabeth J.M. Baltussen, M. Baris Karakullukcu, Behdad Dashtbozorg, Laura A. Smit, Richard Dirven, B.H.W. Hendriks, Henricus J.C.M. Sterenborg, Theo J.M. Ruers Files PDF 121611_1.pdf 6.04 MB Close viewer /islandora/object/uuid:f9467d4d-6f95-47df-baba-7443cf4c237a/datastream/OBJ/view